ABI5 is a gene directly induced to be expressed by ABI3 gene. Since a germination repressing effect of ABI3 is known as a phenomenon occurring by inducing the expression of ABI5, to check if the delay of flowering by ABI3 are caused by an increase in expression of ABI5, the case of overexpressing ABI5 was also checked.
The Arabidopsis DELAY OF GERMINATION 1 gene affects ABSCISIC ACID. INSENSITIVE 5 (ABI5) expression and genetically interacts with
The full-length ABI3 fused to Gal4-BD showed strong autoactivation of the reporter genes (data not shown), as previously reported;41 therefore, trun-cated ABI3 proteins were fused to Gal4-BD and used to evaluate the interaction with abi5-9 and ABI5 (Fig. 3B). As shown previously,27 ABI5 interacted with truncated ABI3 Sequence and Domain Structure of the ABI5 Gene. (A) The sequence displayed corresponds to the reverse complement of nucleotides 33, 132 to 34,991 of … These genes were found on 5 out of 12 chromosomes. StAREB1, StAREB2, StABI5, and StAREB4 were localized on chromosome 1, 4, 9, and 11, respectively. Chromosome 10 contained three loci, including StAREB3, StABL1, and StABL2 (Figure1b). Table 1.
Moreover, the expression of eight other PYL s is also affected during the germination of abi5 mutants. Promoter analysis revealed that an ABI5‐binding region is present next to the TATA box or initiator box. ABI5, an ABA-insensitive gene, encodes a basic leucine zipper transcription factor. Expres- sion of ABI5 defines a narrow developmental checkpoint following germination, during which Arabidopsis plants sense the water status in the environment. ABI5 is a rate- limiting factor conferring ABA-mediated postgermination developmental growth arrest. Ubiquitinated.
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In addition, HY5 binding to the ABI5 2021-01-01 · In Arabidopsis, ABI5 is downstream of the ABA receptor gene PYR/PYL/RCAR and can affect the expression of PYLs (PYL11 and PYL12) through feedback regulation . Overexpression of MdABI5 confers hypersensitivity to ABA in apple calli, and increases the transcription level of MdPYL5 , an ABA sensitive marker homologous to Arabidopsis PYL11 and PYL12 [ 56 ] ( Fig. 5 ). 2013-12-19 · Furthermore, TAP46 interacts with ABI5 in vivo, and overexpression of TAP46 leads to higher abundance of ABI5 in both free form and phosphorylated form, with the concomitant increase in the transcript levels of many downstream genes of ABI5.
By contrast, ABI5 regulates the expression of PYL11 and PYL12 by directly binding to their promoters. Moreover, the expression of eight other PYL s is also affected during the germination of abi5 mutants. Promoter analysis revealed that an ABI5‐binding region is present next to the TATA box or initiator box.
Transcription of ABI3, ABI4 and ABI5 genes is promoted by. ABA in imbibed we used a positional cloning approach to identify the ABI5. The Arabidopsis Abscisic Acid Response Gene AB15 Encodes a Basic Leucine Zipper Transcription Genetic analysis shows that abi5-4 is epistatic toafp-1, indicating the ABA hypersensitivity of afpmutants requires ABI5.
ABI5 regulates the expression of ABA induced, mostly seed-specific, AtEM genes that encode class I late embryogen-esis-abundant (LEA) proteins important for seed maturation (6, 9, 10). Interestingly, the ABI5 gene was also expressed in young seedlings, suggesting that ABI5 may have additional functions
WRKY40 facilitates the recruitment of JMJ17 to the ABI5 chromatin, which removes gene activation marks (H3K4me3) from the ABI5 chromatin, thereby repressing its expression.
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2015-10-01 · ABI5 gene expression in det1. (a) Real-time PCR analysis of ABI5 mRNA levels in Col-0 and det1 dry seeds. (b) ABI5 mRNA levels in Col-0 and det1 seeds imbibed in liquid media in the presence or absence of 2.5 μM ABA for 48 h during cold stratification at 4 °C. Brackets indicate fold change due to ABA treatment. Comparison of seed and ABA-inducible vegetative gene expression in wild-type and abi5-1 plants indicates that ABI5 regulates a subset of late embryogenesis-abundant genes during both developmental stages.
AFP2 mutants affect a number of ABA mediated processes such as germination and response to osmotic and sugar stress.
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The abi5-5 mutation was characterized at the molecular level and was shown to result from a two base pair deletion in the coding sequence of the ABI 5 gene. The wild type and mutant recombinant proteins were produced in E. coli and were assayed for DNA-binding activity on their target promoters by electrophoretic mobility shift assay (EMSA).
Expres- sion of ABI5 defines a narrow developmental checkpoint following germination, during which Arabidopsis plants sense the water status in the environment. ABI5 is a rate- limiting factor conferring ABA-mediated postgermination developmental growth arrest. 2021-03-06 · ABI5 is a direct target gene of BZR1, and modulating the expression of ABI5 by BZR1 plays important roles in regulating the crosstalk between the brassinosteroid and abscisic acid signalling pathways. NF-YC9 mediates abscisic acid (ABA) signaling via targeting to and aiding the ABA-responsive transcription factors such as ABI5. gene is indeed ABI5.